• How to determine tocopherols in different vegetable oils?
  • Herein, rapid, sensitive and precise method for the determination of all type of tocopherols in different vegetable oils includinhg sunflower oil, olive oil and extra virgin olive oil is reported. Reverse Phase High Performance Liquid Chromatography (RP-HPLC) with UV detector was used for tocopherol determination.
  • Do solvents affect tocopherols in seed oils?
  • Tocopherols are the natural antioxidants present in plant oils. Their determination is affected by the technique and solvents used for their extraction. The aim of the study was to determine the tocopherol (¦Á, ¦Ã and ¦Ä) content of two underutilized seed oils (avocado and flaxseed oil) and to see the effects of solvents on their extraction methods.
  • What is the best source of -tocopherol?
  • Method had good limit of detection (LOD) (7ng/g) and reproducibility (C.V% 0.9, 0.8 and 0.4 for ¦Á, ¦Ã and ¦Ä tocopherols, respectively). Result showed the best source for ¦Á-tocopherol was sun flower oil (146.65¡À1.7mg/kg). Oil richest in ¦Ä-tocopherol was olive oil and ¦Ä-tocopherol was absent in extra virgin olive oil.
  • How to extract tocols from grains and oilseeds?
  • However, the solvent extraction with saponification is the most commonly used method for the extraction of tocols from grains, oilseeds, and biological tissues. The degradation of tocols occurs very rapidly during the sample preparation, thus, the tocols must be stabilized with ascorbic acid, butylated hydroxytoluene (BHT) or pyrogallol.
  • Can nano-LC be used to separate tocopherols in vegetable oils?
  • Nano-LC has been applied to the separation of tocopherols in several vegetable oils including avocado, corn, grapeseed, hazelnut, and red palm, using chromolith RP-C 18 capillary column ( Cerretani, Lerma-Garc¨ªa, Herrero-Mart¨ªnez, Gallina-Toschi, & Sim¨®-Alfonso, 2010 ).
  • How to determine the tocopherol content of oil samples?
  • The tocopherol (¦Á, ¦Ã and ¦Ä) contents of oil samples were determined by a Acquity reverse phase UPLC (Ultra performance liquid chromatograph) H-Class (Waters, India) equipped with a Photo diode Array (PDA) detector at 295 nm. A reversed-phase column (Cortex C18, 100 mm ¡Á 2.1 mm; 1.6¦Ìm) was used for the separation of tocopherols, maintained at 35 ¡ãC.